Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: Requirement of p33:p92 interaction for replicase assembly
نویسندگان
چکیده
منابع مشابه
In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70.
To gain insights into the functions of a viral RNA replicase, we have assembled in vitro and entirely from nonplant sources, a fully functional replicase complex of Tomato bushy stunt virus (TBSV). The formation of the TBSV replicase required two purified recombinant TBSV replication proteins, which were obtained from E. coli, the viral RNA replicon, rATP, rGTP, and a yeast cell-free extract. T...
متن کاملNoncanonical Role for the Host Vps4 AAA+ ATPase ESCRT Protein in the Formation of Tomato Bushy Stunt Virus Replicase
Assembling of the membrane-bound viral replicase complexes (VRCs) consisting of viral- and host-encoded proteins is a key step during the replication of positive-stranded RNA viruses in the infected cells. Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host have revealed the involvement of eleven cellular ESCRT (endosomal sorting complexes required for ...
متن کاملPhysicochemical Studies on Tomato Bushy Stunt Virus Variants
Tomato bushy stunt virus particles have previously been physically and chemically well characterized. Different strains, however, are known to have different host plants and symptoms, and they can be distinguished serologically. In an attempt to correlate this behavior with physicochemical properties, we have performed a comparative study on isolates of four of the main variants of this virus (...
متن کاملOn the rigidity of RNA in tomato bushy stunt virus.
The motional state of RNA in tomato bushy stunt rirlls, both in thr crystalline st,attx and in solution, has been invrstigat,ed IuGng 31P nuclear magnetic resonance methods. It, has been found that t’hr RNA is highly immobile in tho nativ Crus. and it is suggested that t,he lack of a high-resolution X-ray diffraction patt,crn for either the RNB or the N-terminal repions of the pro&n coat molcrn...
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هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...
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ژورنال
عنوان ژورنال: Virology
سال: 2006
ISSN: 0042-6822
DOI: 10.1016/j.virol.2005.09.038